induced acute chemical inflammation of synovial membrane and fetlock joint capsule by injecting turpentine oil and gentamycin in equine

clinical observations with gross and microscopic changes of acute chemical inflammation of fetlock joint by injecting turpentine oil and gentamycin in 4 donkeys 2 to 4 years of age and 150 to 200kg/bw were recorded. the animals coordination, pain at the site of injection, local heat joint circumferences and weight bearing after inducing arthritis in fetlock forelimb and hind limb were noted down. o.2ml of turpentine oil and o.8ml of gentamycin was injected to the right forelimb and left hindlimb fetlock joints of animal no.1 whereas 0.1 ml of turpentine oil and o.9ml of gentamycin injected to the right forelimb of animal no.2. injection of 0/1 ml of turpentine oil with 1ml of gentamycin combination to the right forelimb and 0/2ml of the turpentine and 0.8ml of gentamycin to the left hindlimb of animal no.3, whereas 0.lml of tupentine oil and 0.9ml of gentamycin to the right forelimb of animal no 4 to record clinical, gross and microscopic changes in animal no.1 and 2 after 5 days and in animals no.3 and 4 after 21 days of post injection. the severity of inflammation, swelling and lameness were quite evident in animal no.3 after a day of injection as compared to no.1, 2 and 3. local heat, pain at the site with positive flexion test and oozing of exudate at the site of injection on 5 days of injection in animal no 3 was quite significant exudative reaction at the posterior aspect of fetlock with fibrinous inflammation of synovial layer considered to be the major gross changes in animals no 1 and 2, synovitis, exudative fibrinous and synovial pouch, with petechial haemorrhage end increased in thickness of synovial layer and erosion of joint cartilaqe were considered to be the major gross changes of animal no.3. fibrinous inflemmative reaction of synovial surface was observed in animal no.4. the mild inflarnmativa reaction with infiltration of lymphocytes, macrophaqes and plasma cells was observed in animals no.1 and 2, where as hyperplasia and hypertrophy of synovial layer were characteristic microscopic changes in animal no.3.